Gene description for CD9
Gene name CD9 molecule
Gene symbol CD9
Other names/aliases BTCC-1
DRAP-27
MIC3
MRP-1
TSPAN-29
TSPAN29
Species Homo sapiens
 Experiment description of studies that identified CD9 in Melanoma cells

1
Experiment ID617
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameLOX IMVI
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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2
Experiment ID618
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameM14
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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3
Experiment ID622
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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4
Experiment ID625
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleMelanoma cells
Sample nameUACC-62
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
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5
Experiment ID1624
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

6
Experiment ID1625
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

7
Experiment ID1626
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyLC-MS/MS
Western blotting
EV-TRACK -

8
Experiment ID1627
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

9
Experiment ID1628
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Western blotting
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

10
Experiment ID2865
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 30918259   
OrganismHomo sapiens
Experiment descriptionSulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
AuthorsIm EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC.
Journal name Nat Commun
Publication year2019
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID2877
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 30918259   
OrganismHomo sapiens
Experiment descriptionSulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
AuthorsIm EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC.
Journal name Nat Commun
Publication year2019
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID2878
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 30918259   
OrganismHomo sapiens
Experiment descriptionSulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
AuthorsIm EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC.
Journal name Nat Commun
Publication year2019
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID2879
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Western blotting
PubMed ID 30918259   
OrganismHomo sapiens
Experiment descriptionSulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A
AuthorsIm EJ, Lee CH, Moon PG, Rangaswamy GG, Lee B, Lee JM, Lee JC, Jee JG, Bae JS, Kwon TK, Kang KW, Jeong MS, Lee JE, Jung HS, Ro HJ, Jun S, Kang W, Seo SY, Cho YE, Song BJ, Baek MC.
Journal name Nat Commun
Publication year2019
SampleMelanoma cells
Sample nameSK-MEL-28
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

14
Experiment ID1627
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

15
Experiment ID1628
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 34012515   
OrganismHomo sapiens
Experiment descriptionCD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells
AuthorsSuárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M.
Journal name J Extracell Vesicles
Publication year2021
SampleMelanoma cells
Sample nameSK‐MEL‐147
Isolation/purification methodsDifferential centrifugation
Centrifugal ultrafiltration
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Lipid
Methods used in the studyLC-MS/MS
MALDI-MS
Western blotting
EV-TRACK -

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